Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
NADPH | nitrofurazone as electron acceptor | Bacillus subtilis | |
0.0035 | - |
NADPH | FMN as electron accceptor | Bacillus subtilis | |
0.0047 | - |
FMN | - |
Bacillus subtilis | |
0.0163 | - |
nitrofurazone | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Bacillus subtilis |
28320 | - |
calculated from sequence of cDNA | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
ISW 1214. | - |
Bacillus subtilis ISW 1214. | - |
ISW 1214. | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Bacillus subtilis | FMNH2 + NADP+ | - |
? | |
FMN + NADPH | - |
Bacillus subtilis ISW 1214. | FMNH2 + NADP+ | - |
? | |
nitrofurazone + NADPH + 4 H+ | - |
Bacillus subtilis | 5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O | - |
? | |
nitrofurazone + NADPH + 4 H+ | - |
Bacillus subtilis ISW 1214. | 5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
enzyme assay | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Bacillus subtilis | |
NADPH | - |
Bacillus subtilis |